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Image Search Results
Journal: Research in veterinary science
Article Title: In vitro protective efficacy of Lithium chloride against Mycoplasma hyopneumoniae infection.
doi: 10.1016/j.rvsc.2016.03.013
Figure Lengend Snippet: Fig. 1. Antibacterial activity of LiCl against M. hyopneumoniae (1 × 106 CCU/ml) infected PK-cells (1 × 105 cells/well). Cells infected with M. hyopneumoniae, XLW-1 for 10 h and treated with LiCl for 12 h. (A) DNA extracted and quantified by qRT–PCR (log10 copies of DNA/well). Data presented as Means ± SD (n = 3). ⁎p b 0.008 and ⁎⁎p b 0.001 vs. control. (B) M. hyopneumoniae treated with LiCl (direct inactivation) before inoculating into cells. (C) Cells treated with LiCl then infected with M. hyopneumoniae, XLW-1 (pre-infection). DNA was extracted and quantified by qRT–PCR as above. A direct effect on bacteria was observed but not in cells. Data presented as Means ± SD (n = 3) ⁎p b 0.05 and p b 0.54 vs. control. (D) Effect of LiCl treatment was evaluated by IFA. Cells washed, fixed and probed with primary (p46 monoclonal antibody) and secondary-FITC conjugated antibodies. FITC- fluorescence was measured at OD 535. ⁎⁎P b 0.001 vs. control.
Article Snippet: We further infected cells (1 × 105 cells/well) in 96-well plates with M. hyopneumoniae (1 × 106 CFU/ml) at 37 °C for 10 h. Cells washed and inoculated LiCl (10–40 mM) for 12 h. Expression level of M. hyopneumoniae surface antigen (p46) was evaluated by IFA using
Techniques: Activity Assay, Infection, Quantitative RT-PCR, Control, Bacteria
Journal: Briefings in Bioinformatics
Article Title: Integrative approaches based on genomic techniques in the functional studies on enhancers
doi: 10.1093/bib/bbad442
Figure Lengend Snippet: Key questions about enhancers are addressed by different molecular information
Article Snippet:
Techniques: Activity Assay, Sequencing, DNA Methylation Assay, Gene Expression, Expressing, Activation Assay